Gel technology has long found use in proteomics and much research has been done on it over the years. Characterisation of low abundance proteins and separating proteins at extremes of both isoelectric point & molecular weight have historically been a problem when it comes to 2D gels. There are signs that 2D gel image analysis can be used to map proteins with varying abundance but a fully automated system could give further insights on protein expression in different cell types, stages of development & various associated disease states. However, there have been recent advances. For instance, Tecan has come up with ProTeam FFE which is a device combining free flow electrophoresis and 2D gels to improve separation. Therefore better characterisation and visualisation of low abundant proteins can be achieved. Techniques like non-equilibrium pH gradient electrophoresis & strategic modifications to 2D electrophoresis (2DE) have been reported to give better resolution of basic proteins.